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KMID : 0370220070510020088
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Yakhak Hoeji
2007 Volume.51 No. 2 p.88 ~ p.95
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Anti-oxidative Compounds from The Aerial Parts of Atractylodes macrocephala Koidzumi
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Han Jung-Hoon
Chung Sung-Hee
Kim Sung-Gun
Whang Wan-Kyunn
Kim Do-Hoon
Kim Gi-Eun
Kim Jin-Hyo
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Abstract
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Atractylodes macrocephala has been used for renal anorexia, gastroenteritis, cold, dyspepsia in Korean folk medicine. Specially aerial parts has been eaten as edible mountain herbs. In order to investigate the efficacy of antioxidant activity the activity guided fractionation and isolation of physiologically active substance were peformed. For the investigation of the active components from Atractylodes macrocephala MeOH extracts of aerial parts of Atractylodes macrocephala Koidzumi L. were suspended with H_2O, partitioned by CHCl_3. In order to investigate the efficacy of antioxidative activity the activity guided fractionation and isolation of physiologically active substance were peformed. CHCl_3, H_2$O, 30% MeOH, 60% MeOH, MeOH fractions were examined antioxidative activity by DPPH method. It was revealed that 30% MeOH and 60% MeOH fractions have significantly antioxidant activity. From 30% MeOH and 60% MeOH fraction, six flavonoids (7-methoxy-pinocembrin-7-O-{beta}-D-glucopyranoside, apige nin-8-C-{beta}-D-glucopyranoside, 4’’-caffeoyl-luteolin-6-glucopyranoside, luteloin-6-C-{beta}-D-glucopyranoside, apigenin-6-C-{beta}-D-glucopyranoside, luteolin) and four phenylpropanoids (3-feruloylquinic acid, 4,5-di-O-caffeoylquinic acid, feruloyl acid, 3,5-di-O-caffeoylquinic acid) were isolated. To investigate the antioxidant activities of each compounds, we measured radical scavening activity with DPPH method and anti-lipid peroxidative efficacy on low density lipoprotein (LDL) with TBARS assay. Six compounds (III, IV, V, VI, IX, X) which have antioxidant factor showed significant activities.
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KEYWORD
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Atractylodes macrocephala, DPPH method, TBARS assay, anti-lipid peroxidation
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